Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
derived from the bone marrow of a 50-year-old woman with B-acute lymphoblastic leukemia (B-ALL) in 1985; cells were described in the literature to carry the three-way-translocation t(8;14;18) and to overexpress the BCL2 and MYC genes. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Permissions and restrictions:
Morphology:
round to polymorph small cells, growing singly or in clumps in suspension;
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Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximum density at about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 48(44-50)<2n>XX, +8, +12, t(3;4)(q27;p11), der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), ider(8)(q10)t(8;14)(q24;q32)t(14;18)(q32;q21), der(12)t(?1;12)(q25;q24), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - sideline with dup(2)(p1?p2?) - carries rearrangements of BCL2, BCL6, IGH and MYC - resembles published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
