7926
ACC 587
Cell line:
7926
DSMZ no.:
ACC 587
Species:
murine (mouse) (Mus musculus)
Cell type:
acute myeloid leukemia
Origin:
murine radiation-induced acute myeloid leukemia (AML) cell line derived from splenocytes of the C3H inbred mouse strain in 1997; cell line was described in the literature to be radio-sensitive
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
mostly round cells growing in clumps or singly in suspesion
Medium:
80% RPMI 1640 + 20% h.i. FBS + (10 ng/ml mIL-3)
Subculture:
split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; cells are very difficult to culture, mIL-3 supports growth of the cells, cells grow best in 12- or 24-well-plates; after thawing, for the first 7-10 days there is virtually no cell growth or cells grow very slowly, viability may be initially very low, not exceeding 80-85%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-80 hours
Harvest:
maximum density at about 1-1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list