AP-1060
ACC 593
Cell line:
AP-1060
DSMZ no.:
ACC 593
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the bone marrow of a 45-year-old man with acute promyelocytic leukemia (APL = AML FAB M3) in fourth relapse in 1998; cells were immortalized using ethyl-nitrosourea; cells are described in the literature to be constitutively dependent on GM-CSF and IL-3 or G-CSF; cells carry and express the t(15;17)(q22;q11.2) PML-RARA fusion gene and the t(12;15)(p13;q25) ETV6-NTRK3 fusion gene
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
70% Iscove's MDM + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSMZ ACC 35)
Subculture:
cells are difficult to culture! Seed out at ca. 1 x 106 cells/ml in a 24-well-plate; split saturated culture 1:2 or less once or twice a week; maintain at ca. 1.0-1.5 x 106 cells/ml; use always 24- or 12-well-plates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80 hours
Harvest:
maximum density at about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD4+, CD13(+), CD14-, CD15(+), CD19-, CD33+, CD34(+), HLA-DR-, CD68+, cCD3-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 3% polyploidy - 46(42-47)<2n>XY, t(3;14)(p21;q11.2), add(5)(q33), del(6)(q25), add(12)(p13), t(15;17)(q22;q21); carries t(15;17) with rearrangement of PML and RARA; resembles published karyotype
Molec. Genetics:
expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list