JL-1
ACC 596
Cell line:
JL-1
DSMZ no.:
ACC 596
Species:
human (Homo sapiens)
Cell type:
epithelioid mesothelioma
Origin:
established from the untreated malignant epithelioid mesothelioma of the pleura of a 54-year-old man with asbestos cancer in 2003
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% DMEM or NCTC-109 + 20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca.1- 2 x 106 cells/175 cm2; split confluent culture 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109)
Doubling time:
ca. 70-80 hours
Harvest:
cell harvest of ca. 5-7 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded karyotype with 12 % polyploidy; 42-47<2n>XY, +5, +7, -10, -13, +20, der(1)t(1;15)(q11;q11), inv(3)(p1?q2?), der(5)t(5;16)(q11;?), add(10)(q25), der(11)t(10;11)(q21;q23), der(14)t(1;14)(q11;q31); sdl with add(9)(p11), del(16)(q11); karyotype displays significant intercellular differences;
resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list