SCC-4
ACC 618
Cell line:
SCC-4
DSMZ no.:
ACC 618
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from the squamous cell carcinoma of the tongue from a 55-year-old man after radio- and chemotherapy in 1980
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers; image
Medium:
80% mixture of Ham's F12 + DMEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 40 ng/ml hydrocortisone
Subculture:
seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
maximum density at ca. 4 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged flat-moded heteroploid karyotype with pentaploid and decaploid peaks; 100-117/202-266<5N/10N>XXYY/XXXXYYYY, add(Y)(q12), add(2)(q23), add(3)(q28), del(3)(p11), add(4)(p15), ins(4;?)(q32;??), incomplete
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list