HCEC-H9C1

ACC 649

Cell line:

HCEC-H9C1

DSMZ no.:

ACC 649

Species:

human (Homo sapiens)

Cell type:

corneal endothelium

Origin:

HCEC-H9C1 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 (ACC 646) established from normal cells of the posterior epithelium of the cornea of a 91-year-old woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described in the literature to represent developing or transitional cells of the so-called corneal endothelium

Reference(s):

15035, 18144

Biosafety level:

1, GMO-S1

Permissions and restrictions:

A, D

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers; image; image; image

Medium:

Human-Endothelial-SFM + 10 ng/ml FGF-2 (observe the shelf-life of the medium; do not apply h.i. FBS in order to keep differentiation status)

Subculture:

seed out at ca. 0.5 x 10⁶ cells/25 cm² flask; flasks must be coated with 10 µg/ml laminin and 10 mg/ml chondroitin sulfate; split culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (max. 2 min); stop trypsination by using medium containing protease inhibitor cocktail (Sigma P1860) at 500-fold dilution; do not apply antibiotics, cells are highly sensitive to antibiotics (cytotoxic)

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 50-60 hours

Harvest:

cell harvest of ca. 4-5 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line HCEC-12

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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