Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1995 from the pleural effusion of a 85-year-old man with B-NHL (primary effusion lymphoma, PEL) not infected with HIV; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.
Permissions and restrictions:
Morphology:
round cells growing singly and in clusters in suspension;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum densitiy of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, smIgG -, smIgM -, smkappa -, smlambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat moded hyperdiploid karyotype; 47(43-48)<2n>X, -Y, +7, +8, der(1)t(1;16)(q44;q13), del(7)(p21.1p21.2), t(8;15)(p21;q26), del(13)(q13), der(14)t(12;14)(q11;p11),t(16;22)(q13;q12), der(19)t(19;19)(p13.1;q13.3), del(20)(p11.2p11.3);
resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
