HD-MAR-2
ACC 685
Cell line:
HD-MAR-2
DSMZ no.:
ACC 685
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established in 1977 from the pleural effusion of a 20-year-old man with a recurrent T-lymphoblasic non-Hodgkin lymphoma after treatment of the originally diagnosed Hodgkin lymphoma
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml, maintain at ca. 0.2-2 x 106 cells/ml, split saturated culture 1:2 to 1:4 every 2-3 days; upon thawing viability decrease to about 60%, but cells recover within a week; it is recommended to culture the cells with 20% FBS during the first week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype; 92-99<4n>XXYY, -4, +8, +19, +20, +20, +21, +21, +22, +22, del(1)(q21), add(6)(q11)/add(6)(q25), der(9)t(9;10)(p22;?p13)t(9;14)(q35;q11.2)x2, der(14)t(9;14)x2-4, der(17;19)(q10;q10)x2, add(19)(p11), del(19)(q11), del(20)(q11); carries t(9;14) directing upregulation of truncated NOTCH1 by TRA/TRD (TCR@A/D) juxtaposition; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list