Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from peripheral blood mononuclear cells of a 68-year-old woman with mantle cell lymphoma at relapse in 1997; described in the literature to carry a t(11;14)(q13;q32) leading to CCND1-IGH (BCL1-IGH) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
round cells growing singly in suspension;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat moded hypertriploid karyotype; 70-75<3n>XXY, -3, -5, +7, +14, -16, -16, -18, -19, +20, +20, -21, +4mar, del(3)(q13)x1-2, del(6)(22), i(7)(p10), der(8)t(8;14)(q24;q32)t(14;11)(q32;q13)x2, t(8;14)(q24;q32)x2, der(11)t(11;14)(q2?;q11)t(14;8)(q32;q24)x1-2, der(11)t(11;14)(q13;q32)x2, add(14)(q21), der(13)t(3;13)(q25;q32)x1-2; carries complex rearrangements of IGH with MYC and CCND1 (BCL1) consistent with MCL; although presence of Y-chromosome is inconsistent with published patient gender, karyotype otherwise resembles that published
Molec. Genetics:
expression of fusion gene IGH-CCND1 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
