Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established 1988 from a 41-year-old man with acute myeloid leukemia (AML-M7) during refractory relapse initially presenting with acute myelofibrosis. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round cells growing singly in suspension; occasionally semi adherent cells and a few giant cells are present;
image;
image;
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Medium:
80% RPMI 1640 + 20% h.i. FBS + 5 ng/ml GM-CSF
Subculture:
seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.8-3 x 106 cells/ml; split culture 1:3 to 1:8 every 3-5 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
maximum density at ca. 5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 8% polyploidy; 42(40-43)<2n>X, -Y, -7, -14, -17, del(5)(q13q32), der(6)t(6;7)(p23;p11), i(7)(q10), der(12)t(12;14)(p12;q11), add(20)(q13)x1-2; matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
