KYAE-1
ACC 703
Cell line:
KYAE-1
DSMZ no.:
ACC 703
Species:
human (Homo sapiens)
Cell type:
esophageal adenocarcinoma
Origin:
established from the pleural effusion of a 60-year-old man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a TP53 mutation (C455T)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelial cells growing adherently as monolayer and small clusters
Medium:
40% Ham's F12 + 40% RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; cells do not grow to confluency; split sub-confluent culture 1:2 to 1:5 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list