KASUMI-3
ACC 714
Cell line:
KASUMI-3
DSMZ no.:
ACC 714
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from blast cells of the bone marrow of a 57-year-old man with minimally differentiated acute myeloblasic leukemia (AML M0); cells were described in the literature to carry a t(3;7) leading to alteration of MECOM-TRB (EVI1-TRB) genes and to MECOM (EVI1) overexpression
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
start with ca. 0.5-1 x 106 cells/ml in 24-well plates; later, maintain at ca. 0.3-1 x 106 cells/ml in flasks; split saturated culture 1:2 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 -, cy/smCD3 -, CD4 +, CD5 -, CD7 +, CD8 -, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype with 9% polyploidy; 46(43-47)<2n>XY, der(2)t(2;12)(p15;p13), der(3)t(3;7)(q26;q35)t(7;8)(q22;q23), der(4)t(4;8)(q13;q21), del(5)(q15), der(5)t(5;10)(q33;q24), der(7)t(3;7)(q26;q35)del(7)(p1?), der(8)t(4;8)(q13;q12), del(9)(q32), del(10)(q24), add(12)(p11), add(16)(q13.2) ; carries rearrangement of MECOM (EVI1) with TRB (TCRB); resembles published karyotypes
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list