Species:
human (Homo sapiens)
Cell type:
acute megakaryoblastic leukemia
Origin:
derived from a woman with acute megakaryocytic leukemia (AML M7); cell line expresses the SET-NUP214 fusion transcript. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in 24-well plate; cells may grow slowly in the first week after thawing; split culture 1:2 twice a week; cells are difficult to culture
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 +, CD71 +, CD235a -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 49(45-52)<2n>XX, -8, +13, +13, -15, -16, -17, +5 mar, del(2)(q11q22), t(2;15)(p16;q14), der(6)del(6)(p23)t(6;17)(q27;q11), del(9)(q34.11q34.13), ins(13;16)(q31;q13q23), ?r(13)(q31q32)x3, der(14)t(13;14)(p12;q12)t(14;9)(q21;q11), del(16)(q13q23), der(19)t(15;19)(q?;p13); karyotype hitherto unreported; carries cryptic 9q34 deletion effecting SET-NUP214 fusion; rearrangements at 13q31 associated with activation of miR-17~92 cluster
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
