MDA-MB-468
ACC 738
Cell line:
MDA-MB-468
DSMZ no.:
ACC 738
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established in 1977 from the pleural effusion of a 51-year-old woman with invasive breast carcinoma; cells are described in the literature to carry a p53 point mutation at codon 273 leading to Arg-His substitution and an ampliefied EGFR; triple-negative; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80-90% Leibovitz´ L15 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed)
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 15-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
triple-negative confirmed by absence of ER, PR, AR and HER2 in Western blots
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list