MUG-CHOR-1
ACC 755
Cell line:
MUG-CHOR-1
DSMZ no.:
ACC 755
Species:
human (Homo sapiens)
Cell type:
chordoma
Origin:
established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old woman
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in monolayers
Medium:
80% mixture of Iscove's MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 75-100 hours
Harvest:
cell harvest of ca. 4 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list