I83-E95
ACC 763
Cell line:
I83-E95
DSMZ no.:
ACC 763
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1994 from the peripheral blood of a 75-year-old man with chronic lymphocytic leukemia (CLL, Binet A stage at diagnosis) and subjected to immortalization by Epstein-Barr virus; EBV+ B-lymphoblastoid sister cell line (B-LCL) I83-LCL is available (ACC 764)
Biosafety level:
2
Risk assessment:
The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
small to medium sized irregularly shaped cells growing singly or in clumps
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 every 2-3 days; cells grow better in 24-well plates than in culture flasks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-60 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 +, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype; 83-88<4n>XXYY, -6, -14, -18, der(2)t(2;11)(q12;q25)x2, der(11)t(2;11)(q12;q13)x2, der(13)t(13;13)(p11;q14)x2, der(14)t(14;14)(q32;q22)x2, der(22)t(13;22)(q31;p11)x2; carries chromosome rearrangements leading to loss of 13q11-14; resembles published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list