WA-C3CD5+
ACC 769
Cell line:
WA-C3CD5+
DSMZ no.:
ACC 769
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1994 from the peripheral blood leukocytes of a man with B-type chronic lymphocytic leukemia (CLL, Rai stage I at diagnosis); CD5 positive sister cell line of WA-OSEL (DSM ACC 767)
Biosafety level:
2
Risk assessment:
The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing singly and in small clusters in suspension
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 every two days; maintain at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 - 50 hours
Harvest:
maximum density at ca. 0.7-1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 6% polyploidy;43-51<2n>XY, +9, +13, +21, der(6)t(6;9)(q14;q21), der(7)t(7;17)(q10;q10), del(13)(q14q21)x2, del(13)(q14.2q14.3);
nullisomy for DLEU2 locus via focal chr. 13 deletion associated with CLL; marginally related to published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list