MBU-T
ACC 799
Cell line:
MBU-T
DSMZ no.:
ACC 799
Species:
human (Homo sapiens)
Cell type:
keratinocytes
Origin:
established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase, SV40 early region, and epitope-tagged mutated HRAS(V12) linked to IRES-driven GFP expression
Reference(s):
Biosafety level:
2, GMO-S2
Risk assessment:
Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract
Subculture:
seed out at ca. 0.5 - 1 x 106 cells/80 cm2 flask; split 1:2 - 1:4 every 3-4 days using Accutase
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 4-6 x 106 cells/80 cm2
Storage:
frozen with 90% Keratinocyte SFM, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list