MCC-60
ACC 815
Cell line:
MCC-60
DSMZ no.:
ACC 815
Species:
human (Homo sapiens)
Cell type:
colorectal cancer
Origin:
established in 2016 from the primary adenocarcinoma (TNM stage pT4b, pN1a, pM1; tumor grade G3; resection status R0) of the ascending colon of a 65-year-old man
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in monolayers
Medium:
80-85% DMEM + 15-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:4 once a week using trypsin/EDTA; if the cells grow slowly in the first week after thawing, change medium only
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 70-80 hours
Harvest:
cell harvest of ca. 5-7 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list