IPH-926
ACC 827
Cell line:
IPH-926
DSMZ no.:
ACC 827
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from malignant ascites of a metastatic lobular breast cancer of a 72-year-old woman after chemotherapy (tamoxifen) and radiotherapy; cells are described in literature to carry a CDH1 mutation (lack of E-Cadherin expression). Primary tumor cells were ER-positive but others suggested as triple-negative. Due to a mutation in the TP53 gene cells are temperature sensitive. Further described to overexpress the MDR1/ABCB1 drug transporter, to lack beta-catenin expression, to be hyperdiploid and tumorigenic in SCID mice; RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS, containing additionally 10 mM HEPES, 2.5 g/l glucose, 2 mM L-glutamine, 1 mM pyruvate, 10 µg/ml insulin
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 every 5-10 days using trypsin/EDTA; due to the temperature sensitivity cells should be kept
and handled whenever possible at 37°C; medium should be pre-warmed before use
Incubation:
obligatory at 37 °C with 5% CO2
Doubling time:
in the first 1-2 weeks about 200 hours then 80-120 hours
Harvest:
ca. 15-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
triple negative not confirmed: expression of HER2, while absence of ER, AR and PR in Western blots
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list