JVE-367
ACC 829
Cell line:
JVE-367
DSMZ no.:
ACC 829
Species:
human (Homo sapiens)
Cell type:
neuroendocrine cancer
Origin:
established from the primary tissue of the ileocecal junction of the colon of a 61-year-old woman with large cell neuroendocrine malignant carcinoma (Dukes stage C1); cells were described in literature to carry a mutation in the BRAF gene and a hypermethylation of the CDKN2A promoter
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round and polymorphic cells growing in suspension with the formation of large clusters; clusters may be difficult to disperse
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/25 cm2 flask;
split culture 1:2 once or twice a week (trypsin/EDTA is not necessary)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 4-5 days
Harvest:
cell harvest of 10-13 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list