HEK293-DR-GFP
ACC 844
Cell line:
HEK293-DR-GFP
DSMZ no.:
ACC 844
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
Derivative of the modified human embryonal kidney cell line 293 Tet-Off (Clontech) as reporter cell line for studying chromosomal double-strand repair (DR). The cell line contains the hprtDR-GFP reporter and is puromycin resistant. A series of modified cell lines with disruptions in RAD51 paralogs are also available (DSMZ ACC 845-ACC 854).
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin)
Subculture:
culture vessels should be coated with 0.01%
poly-L-lysine solution; seed out at 1 x 106 cells/25 cm2; split subconfluent culture 1:3 to 1:4 every 2-3 days using trypsin/EDTA; optimal density around 4 x 106 cells/25 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 8 x 106 cells/25 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list