MUG-MEL2
ACC 855
Cell line:
MUG-MEL2
DSMZ no.:
ACC 855
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established in 2014 from a cutaneous metastasis of a 48-year-old man with highly aggressive ulcerated melanoma after chemotherapy. Cells are described to be moderately hyperdiploid, carry a mutation in the N-Ras-gene (pQ61R) but not in the BRAF. Further described to be tumorigenic in nude mice and able to grow in 3D using hydrogel
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
elongate or triangular dendritic cells growing adherently as monolayer; cells are highly pigmented and sedimented cells have a brown color
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM glutamine
Subculture:
seed out at 1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61R mutation (mu/wt), but no BRAF V600 mutation
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list