COR-L23
ACC 882
Cell line:
COR-L23
DSMZ no.:
ACC 882
Species:
human (Homo sapiens)
Cell type:
large cell lung carcinoma
Origin:
established in 1985 from a pleural effusion
of a 62-year-old male with a giant cell anaplastic carcinoma of the lung; described to have a c-myc amplification, a kras mutation and to form tumors in nude mice. Alias name: COR-L23P (P=parental); cells are sensitive towards doxorubicin and a multidrug-resistant subclone is available (COR-L23R, ACC 883); listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 1 x 106 cells in a 175 cm2 flask; split semi-confluent 1:5 - 1:10 twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 10-12 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list