HCC-1599BL

human (Homo sapiens)

B lymphoblastoid cells

lymphoblastoid cell line established in 1995 from peripheral blood of a 44-year-old woman with ductal breast carcinoma; autologous carcinoma cell line HCC-1599 is available (DSMZ ACC 503)

1

The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.

A

round to polymorphic cells growing singly and partly in aggregates in suspension; image

90% RPMI 1640 + 10% h.i. FBS

seed out at about 0.3-0.5 x 10⁶ cells/ml; maintain at 0.3-0.8 x 10⁶ cells/ml, split 1:2 to 1:3 every 2-3 days

at 37 °C with 5% CO₂

about 48-72 hours

maximal density of 0.8 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in PCR assay

CD3 -, CD10 -, CD13 -, CD19 (+), CD20 +, CD34 -, CD37 (+), cyCD79a +, CD80 +, HLA-DR +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the autologous cell line HCC-1599

PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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