TLBR-3
ACC 906
Cell line:
TLBR-3
DSMZ no.:
ACC 906
Species:
human (Homo sapiens)
Cell type:
anaplastic large cell lymphoma
Origin:
established in 2011 from primary tumor tissue of a breast implant-associated T-cell anaplastic large cell lymphoma of a 45-year-old-woman; cells are IL-2 dependent and are reported to secrete several cytokines. ALK-negative and up-regulation of survivin, BCL2, NOTCH-1 and NOTCH-1 receptors; sensitive to doxorubicin and honokiol (a SHP-1 activator)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS + 10ng/ml IL-2
Subculture:
seed out at about 0.5 x 106 cells/ml; maintain at 0.2-1 x 106 cells/ml; split 1:3 to 1:8 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20-50 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 +, CD5 -, CD8 -, CD10 -, CD20 -, CD21 -, CD25 +, CD30 +, CD71 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
confirmed triploidy; resembles published karyotype with varying gains and losses of single chromosomes
Molec. Genetics:
absence of fusion gene NPM1-ALK confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list