BL-30
ACC 916
Cell line:
BL-30
DSMZ no.:
ACC 916
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1982 from bone marrow of a 19-year-old male with Burkitt lymphoma in relapse; cells are EBV-negative, contain a mutation in exon 6 of TP53 and were described to carry a t(8;14) translocation; a matched B-lymphoblastoid cell line is available (IARC-139, DSMZ ACC 924)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
95% RPMI 1640 + 5% h.i. FBS
Subculture:
seed out at about 0.1-0.5 x 106 cells/ml; split 1:5 to 1:10 every 2-4 days and maintain at 0.2-0.5 x 106 cells/ml (densities above 1 x 106 cells/ml will induce apoptosis)
Incubation:
at 37 °C with 5% CO2
Doubling time:
varies between 15-24 hours depending on cell density
Harvest:
maximal density of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cy IgM +, sm/cy IgG -, sm/cy kappa -, sm/cy lambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database; matching autologous IARC-139.
Molec. Genetics:
a TP53 mutation in exon 6 (737T>C) was verfied by RT-PCR (while the corresponding B-LCL is wild type)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list