SPI-802 - Discontinued
ACC 92
Cell line:
SPI-802 - Discontinued
DSMZ no.:
ACC 92
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis (cell line K-562)
Origin:
Caution: STR analysis demonstrated that cell line SPI-802 is genetically identical to human chronic myeloid leukemia celll line K-562 (ACC 10). SPI-802 must not be used as model for human T-ALL.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Harvest:
maximal density at about 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
CD3 -, CD13 +, CD19 -, CD33 +, CD71 +, CD235a +, HLAclassI -; image
Authenticity:
STR analysis resulted in the STR profile of cell line K-562.
Cytogenetics:
human hypotriploid karyotype with sharp mode - 60-66<3n>XX, -X, -4, -5, -8, -10, -13, -16, -20, -21, -22, -22, +5mar, der(3)t(3;?)(q22;?), del(4)(q22), del(6)(q15)x2, der(2q11q)t(2;11)(2p11p;2q11q), der(15)t(15;18)(q21;q11/12), der(17)t(17;?)(p11/13;?), der(18)t(18;?)(p11;?), del(20)(q11/12), del(X)(p22) - results of FISH analysis show that one or two marker chromosomes probably derive from Ph
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list