History:
<- J. Neufeld and A. Umbach; {2023} <- J. Neufeld and L. Sauder, Univ. Waterloo, Canada; AQ6F {2014}
Summary and
additional information:
<- J. Neufeld and A. Umbach; {2023} <- J. Neufeld and L. Sauder, Univ. Waterloo, Canada; AQ6F {2014}. Published as "Candidatus Nitrosotenuis aquarius" Sauder et al. 2018. Proposed renaming to "Candidatus Nitrosotenuis aquariorum" Oren et al. 2020. Freshwater aquarium biofilter. Canada, Ontario, Waterloo, University of Waterloo. Taxonomy/description (37580, 40844). Sequence accession no. complete genome: CP024808, 16S rRNA gene: KX023321, BioProject: PRJNA406986, BioSample: SAMN07637255, amoA gene: KX034182. Chemolithoautotrophic. Oxidation of ammonia to nitrite. Risk Group 1: enrichment of Archaea with partly known bacteria (non-specific activity, German BioStoffV Section 2 point (8)), such as <i>Methylibium</i> sp., <i>Reyranella</i> sp., <i>Mesorhizobium</i> sp. (Risk Group 1, German TRBA 466). (Medium 1845, incubation time: more than 4 weeks, pH 8.4, 28°C, aerobic). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months
